SCREENING OF RHIZOBACTERIA FROM ONION RHIZOSPHERE CAN INDUCE SYSTEMIC RESISTANCE TO BACTERIAL LEAF BLIGHT DISEASE ON ONION PLANTS

Milda Ernita, Trimurti Habazar, Nasrun and Jamsari

Abstract


In modern cultivation processes indiscriminate use of pesticides and fertilizers, has led to substantial pollution of soil, air and water. So,  there is an urgent need  to solve the problem. Rhizobacteria are bacteria that colonize plant roots, and these bacteria are known to stimulate growth  and thereby reduce incidence of plant disease by direct and indirect mechanisms. A total of 136 rhizobacteria isolates  were isolated from different rhizosphere soils in central areas of production of onions in Indonesia. These isolates were screened for their capability to enhance  growth and protect  onions against  bacterial leaf blight disease-caused by Xanthomonas axonopodis pv.allii. The results showed that ten isolates can  enhance  growth and protect  onions against bacterial leaf blight diseases.  Five isolates were isolated from West Sumatra, four isolates from Java and one isolated from  North Sumatra.  All  isolates produced indol-3-acetic acid with different concentrations. Molecular identification  of ten isolates belong to  Bacillus sp, Pseudomonas sp, Stenotrophomonas sp and Serratia sp.

Keywords


rhizosphere bacteria; bacterial leaf blight disease; onion

Full Text:

PDF

References


Alvares, A.M., Buddenhagen, I.W., Buddenhagen,E.S. and Domen, H.Y. 1978. Bacterial blight of onion, a new disease caused by a Xanthomonas sp. phytopathology. 68:1132-1136.

Ahemad, M. and M. Kibret. 2014. Mechanisms and applications of plant growth promoting rhizobacteria. J. of King Saud University- Science. 26: pp1-20

Ahmad F. I., I. Ahmad I, and Khan MS. 2005. Indole Acetil acid production by the indigenous isolates of Azotobacter and Flourescent Pseudomonas in the presence and absence of tryptophan. J..Biology Turkey. 29: pp 29-34.

Arshad, M. and Frankenberger, Jr.W.T. 1993. Microbial production of plant growth regulators. In Blaine F. and metting, Jr. (Eds) Soil Microbial Ecology. Marcel and Dekker, Inc. New York, pp307-347.

Barazani, O. and Friedman, J. 2000. Effect of exogeneously applied L-tryptophan on allelochemical activity of plant growth promoting rhizobacteria (PGPR). J. Chem. Ecol. 26, 343-349.

Brimecombe,M.J., F.A. Leij. and J.M. Lynch.2001. The effect of root exudates on rhizospheremicrobial communities. I. The Rhizosphere. Biochemistry and organic subatance at soil-plant interface. Maecell Dekker. New York. pp 95-140

Chakraborty,U., B.N. Chakraborty, A.P.Chakraborty, K. Sunar and P.L. Dey. 2013. Plant growth promoting rhizobacteria mediated improvement of health status of tea plants. Indian J of Biotechnology. Vol 12, January, pp 20-31

Dajtnika, I. 2012. Seleksi bakteri antagonis untuk mengendalikan layu fusarium pada tanaman Phalaenopsis. J. Hortikultura vol 22 (3): 276-284

Fadhli. 2005. Uji tingkat serangan penyakit hawar daun bakteri pada beberapa varietas bawang merah di Kecamatan Lembah Gumanti Kabupaten Solok. Skripsi fakultas Pertanian Unand. Padang

Gent, D.H., J.M. Lang, H.F. Schwartz. 2005. Epiphytic survival of Xanthomonas axonopodis pv.allii and X. axonopodis pv.phaseoli on leguminous hosts and onion. The American Phytopathological Society. Plant Disease, 89(6):558-564

Gent , D. H. And H. F. Schwartz. 2005. Effect of Nitrogen fertilization and Seed contamination on epiphytic populations of Xanthomonas axonopodis pv.alii and development leaf blight of onion. Online. Plant Health Progress doi: 10.12094/PHP-2005-0331-010-RS.

Habazar, T. 2006. Pengenalan penyakit hawar daun bakteri oleh Xanthomonas axonopodis pv.allii pada tanaman bawang merah. Makalah dalam apresiasi penanggulangan OPT tanaman sayuran. 3-6 Oktober di Nganjuk.

Hayat, R.,S. Ali, U. Amara, R. Khalid and I. Ahmed. 2010. Soil beneficial bacteria and their role in plant growth promotion. Ann. Microbiology. DOI 10.1007/s13213-010-0117-1.

Husna, R. 2006. Tingkat serangan penyakit hawar daun bakteri disebabkan oleh Xanthomonas axonopodis pv.allii pada beberapa jenis tanaman bawang (Allium sp). Skripsi. Fakultas Pertanian Unand.Padang

Khan, M.S. 2006. Screening of Free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbio J. 163: 173-181

Klement, Z., K. Rudolph, D.C. Sand. 1990. Methods in Phytobacteriology. Budapest: Akademiai Kiado.

Kloepper ,J. W. and M. N. Scroth. 1981. Relationship of in vitro antibiosis of plant growth promoting rhizobacteria to plant growth and the displacement of root microflora. Phytopathology 71: 1020-1024

Lang, J. M., D.H. Gent, H.F. Schwartz. 2007. Management of xanthomonas leaf blight of onion with bacteriophages and a plant activator. The American Phytophatology Society. Plant Disease.91(7): 871-878

Lugtenberg, B. and F.Kamilova, 2009. Plant growth promoting rhizobacteria. Annual Rev. Microbiology. 63:541-556

Manoharachary, C. and K.G. Mukerji. 2006. Rhizosphere Biology- An Overview. In Soil Biology. Springer –Verlag Berlin Heidelberg. (7). 1-15p

Nelson, L.M. 2004. Plant Growth promoting rhizobacteria (PGPR): prospect for new inoculants. Online. Crop management doi: 10.1094/CM-2004-0301-05-RV.

Patten, C.L and B.R Glick.1996. Bacterial biosynthesis of indole-3-acetic acid. Canadian J. Microbiology. 42; 207-220

Patten, C. and B. Glick. 2002. Role of Pseudomonas putida indolacetic acid in development of the host plant root system. Appl. Environment Microbiology. 68; 3795-3801.

Paulraj, L. and L.W. O'Garro. 1993. Leaf blight of onion in Barbados caused by xanthomonas campestris. Plant Disease. 86. 330-335

Roumagnac, P., Gagnevin, L., Gardan, L., Sutra, L., Manceau, C., Dickstein, E. R., Jones, J. B., Rott, P., dan Pruvost, O. 2004. Polyphasic characterization of Xanthomonads isolated from onion, garlic and welsh onion (Allium spp) and their relatedness to different Xanthomonas species. Int J. Syst . Evol. Microbiology 54:15-24.

Singh, G and K.G. Mukerji. 2006. Root Exudates as Determinant of Rhizospheric Microbial Biodiversity. In Soil Biology. Springer-Verlag Berlin Heidelberg (7); 39-53pp

Sutariati,G.A.K and A. Wahab. 2010. Isolasi dan uji kemampuan rizobakteri indigenous sebagai agensia pengendali hayati penyakit tanaman cabai. J. Hortikultura. Vol 20 (1):86-95

Taufiq,M.A., A. Wahab dan S.H.Hidayat. 2010. Mekanisme ketahanan terinduksi oleh plant growth promoting rhizobacteria (PGPR) pada tanaman cabai terinfeksi cucumber mozaik virus (CMV). J. Jortikultura. Vol 20 (3):274-283

Van Loon, L.C. and P.A.H.M Bakker. 2003. Signalling in Rhizobacteria-Plant Interactions. Ecological studies. Vol 168. Springer-Verlag Berlin Heidelberg. 297-330

Van Loon,L.C., PAHM. Bakker and CMJ. Piterse. 2002. Prospect and challenges for practical application of rhizobacteria-mediated induced systemic resistance. In Induced Resistance in Plants Against Insects and Diseases. IOBC/wprs Bull. Vol 25 (6). 75-82pp

Vessey, J. K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil. 255:571-586


Refbacks

  • There are currently no refbacks.





IJAC: International Journal of Agricultural Science is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.View My Stats